上海M5655 代理 益启供
特点及优点：***，批间稳定性高 产品齐全 涵盖常规染色和ICC实验 口碑良好 客户使用率高
应用：对细胞表面或内部细胞器染色 便于观察细胞的健康活力 评估细胞状态
Use as a colorimetric metabolic activity indicator in cell viability assays.
Thiazolyl Blue Tetrazolium Blue (MTT) may be used in measurement of cell proliferation.MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm. MTT has been used as a histochemical/cytochemical reagent and for the detection of NAD. ADP-linked enzyme systems in tissue cannot be detected with MTT, due to binding of the cation by the cyanide trap used. MTT is rapidly reduced to the formazan, which chelates with nickel, copper, and cobalt; the cobalt chelate has been used in oxidative systems.
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